Sentence examples for from three leaves from inspiring English sources
To obtain homogeneous samples, samples from three leaves of 6 to 8 week-old N. tabacum plants were pooled for each treatment.
Samples were collected 4, 8, 12, 16, and 20 days after inoculation from three leaves of each seedling.
At 12 hours and 24 hours post Xcc infiltration, five leaf discs from three leaves per seedling were cut using a circular 5-mm-diameter cork borer.
For qRT-PCR analysis of GFP gene expression in transgenic T0 plants, total RNAs were isolated individually from three leaves of 20 lines randomly selected from one batch of transformation with each vector indicated in Figure 3A.
Protein extracts from three leaves infiltrated with 5 μM XacPNP in 50 mM Tris as well as control leaves infiltrated with 50 mM Tris both for 30 minutes were prepared by pulverization of leaves in liquid nitrogen followed by re-suspension in 50 mM Hepes-KOH buffer pH 7.5, 330 mM sorbitol, 5 mM sodium ascorbate, 2 mM EDTA, 1 mM MgCl2, 1 mM MnCl2 and 0.33 mM PMSF in a 1 2 ratio.
At 0 and 4 days post inoculation (dpi), leaf discs from ten leaves were collected from each genotype, surface-sterilized with 75% ethanol, rinsed in sterile water, and ground in sterile water for bacteria extraction.
Roger Schmidt's team have had a mediocre start to the season (seven points from five leaves them in 10th place) and are one of the early under-achievers relative to their considerable talent.
Katie Naplatarski, a twelve-year resident of Lorimer street who lives half a block from Five Leaves and Lokal, leaned into the podium, her long hair falling into her face as she read her prepared statement.
A fourth defeat from five leaves them well off the pace at the top of the table, placing even greater emphasis on their Challenge Cup clash at Bradford next Saturday.
Such yeasts were isolated from two leaves of paddy rice (Figure 2A, B).
Leaf surfaces from five leaves in each taxon were scraped by a razor blade, stained with 1 % Safranin-O and dehydrated by concentration series of ethanol.
