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For each assayed tomato sample, extracts from three leaf disks prepared as above were collected and analyzed in adjacent wells of ELISA plates.
The values obtained from three leaf extracts (Table 5) were significantly different (p < 0.05), but the order were inconsistent with the DPPH and ABTS assays.
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Some terrestrial species throw a thread of mucus which they use as a rope to climb from one leaf to another.
We argue that transmission of vibrational signals from one leaf to another via air may be a common phenomenon.
Our measurements also revealed a significant increase in dominant frequency (from 200 to 250 Hz) when vibrational signals were transmitted through air from one leaf to another.
Indeed, whereas A. decemarticulatus workers recruit nestmates mainly after contact with alien insects that have damaged leaf blades [32], Z. annulosus nymphs detect prey visually (even from one leaf to another) and immediately capture them.
Our measurements showed that vibrations are transmitted from one leaf to another even when they were separated by a gap of 11 cm and that females responded to males up to a gap width of 6 cm.
When Z. annulosus nymphs moved from one leaf to another they walked on the upper part of the stems, avoiding putting their legs on the trap where A. decemarticulatus workers ambushed in a group.
Protein was extracted randomly from five leaf disks.
DNA was extracted from eight leaf punches using the MagAttract 96 well DNA kit (QIAGEN, Hilden, Germany).
For non-sibling larvae, we find no difference in survival between groups that come from one leaf fragment and groups that come from ten leaf fragments (Fig. 5) even though the groups of non-sibling larvae that come from one leaf fragment might contain some sibling individuals.
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