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From this sequence we were able to call 15,622,848 variants and found 40 GO terms with significantly different representation in fixed SNPs tagged as non-synonymous versus synonymous.
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Using deep sequencing, we were able to recover and assemble near full-length genome sequences of the 2009 H1N1 virus from two individual patient specimens.
From the sequence analysis, we were able to predict the ORF regions in only 30% of them (results not shown).
We were able to sequence 1 kb amplicons generated from GC-rich regions in our targeted sequences.
Of the six samples that were heterozygous for SQD1, we were able to sequence five haplotypes from three individuals.
We were able to sequence five duplication breakpoints from the set of 24 duplications in Table 1.
However, although we were able to sequence a few distinct haplotypes from heterozygous plants, this was largely unsuccessful.
We were able to read sequences from some of the PCR bands that contained two sequences, but were unable to read sequences from several other bands.
Followed the segregation rule, we were able to distinguish allelic sequences from homoeologous sequences even just having one nucleotide variation between them using the integrated RFAPtools.
Because of its greater phylogenetic distance, we were able to obtain sequences from only a portion of the examined loci from the D. magna genome sequence.
We were able to predict this region from the genomic DNA sequence based on sequence similarity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com