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Poorly aligned regions from this alignment were removed with the Gblocks 0.91b program [ 74], using the default settings, except that allowable gap position was selected to half.
Using the PVS, a consensus sequence was also obtained from this alignment that also search for the variable site with a threshold level of less than or equal to 1.
Putative SNPs were identified from this alignment by extracting single-base-pair mismatches.
Starting from this alignment, a set of 10 full-atoms models was generated for each human monomer.
The 566 amino acid consensus sequence, named HA1-con, was generated from this alignment by using the most common amino acid at each position.
From this alignment, a maximum likelihood analysis using the WAG model [24] was conducted using RAxML [25] with a bootstrap analysis [26] based on 100 replicates.
This extended homeodomain was extracted for each of the eight D. melanogaster Hox-proteins, aligned in AlnEdit using Muscle (see Appendix S3 for the extended homoedomain alignment) and a global HMM was derived from this alignment using HMMer.
The free-energy released from this alignment is then calculated.
Third, we constructed a phylogenetic tree from this alignment.
From this alignment, we derived a total number of 13,324 SNP loci.
From this alignment, phylogenetic trees were constructed by neighbor-joining and maximum likelihood methods.
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