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Supernatants from the two steps were pooled together and 1 ml of the solution from each mouse was subjected to ultracentrifugation at 100,000 × g for 1 h.
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The supernatants from the two extraction steps were pooled (soluble fraction).
Supernatants from the two homogenization steps were pooled, centrifuged at 750 g for 10 min twice.
Supernatants from two extraction steps were pooled and evaporated until dryness in a vacuum concentrator (CentriVap Centrifugal Concentrator, Labconco, Kansas City, MO, USA) at 30°C.
The last two steps were repeated five times and cells were pooled.
Supernatants from the two extractions were pooled.
Fractions from the two peaks were pooled separately.
The data from the three surveys were pooled for analysis.
Therefore results from the two control groups were pooled.
The data from the three observation periods were pooled.
† Data from the three treatment arms were pooled.
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