From the total set of Prx markers a random sample, without replacement, was taken and these were arranged into clusters using the 'gap size' of 5 cM.
Gowinda randomly samples SNPs from the total set of SNPs and records the associated genes.
By sequencing clones derived from RNA from different sources, and/or by normalizing cDNA libraries, the total set of genes sampled can be maximized.
This represents the total set of transcripts from the assembly.
Extrapolating from the sample set, we would estimate that application of this review process to our total set of 608 manual 'unknown' annotations that have corresponding InterPro predictions would allow us to update 145 of the annotations.
In total, tumor copy number on chromosome 1-22 and chromosome X were successfully measured in 481 TCGA breast tumor samples, and normalized gene expression data from the same set of samples were downloaded from TCGA.
Absolute fluorescence readings were background subtracted, averaged for the replicate set of samples from a total of four independent experiments for statistical comparison.
From the total study sample set of 8,700, forty samples were from repeat smears from the same women and were excluded, leaving 8,660 independent samples.
Among the total 612 protein hits found from the three sets of samples, the prophet false discovery rate (FDR) for proteins was at 0.1%.
For each stage, three sets of total RNA samples were extracted independently, and then RNA pool was constructed by mixing aliquot of RNA from the three sets of RNA samples.
Statistical significance of functional groups was assessed using non-parametric Kruskal-Wallis tests and Dunn's Multiple Comparison [ 20] tests (alpha = 0.2), after randomly sampling three hundred sequences from each group, and from the total population to form the sample sets.
