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Briefly, periodontal ligament tissues were gently separated from the surface of the root and digested in the solution of collagenase type I (Worthington Biochem) and dispase (Roche) for 1 h at 37 °C.
The fungus forms an extensive underground network of hyphae that radiate outward from the surface of the root sheath, effectively increasing the surface area for nutrient absorption.
Of great importance is the removal of biofilms or plaque from the surface of the root [6], which can be done using EDTA gels [7] or erbium-doped:yttrium, aluminum, and garnet (Er:YAG) laser [8].
Root apical papilla was gently separated from the surface of the root, minced and digested in a solution of 3 mg/ml collagenase type I (Worthington Biochemicals Corp., Freehold, NJ) and 4 mg/ml dispase (Roche Diagnostic/Boehringer Mannheim Corp., Indianapolis, IN) for 30 minutes at 37°C.
Periodontal ligament tissue was gently separated from the surface of the root and then digested in DMEM containing 1 mg/mL collagenase type I (Worthington, Lakewood, NJ) overnight.
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The fungus and the plant forms structures called ectomycorrhizae, a specialized sheath of hyphae on the surface of the root from which hyphae extend into the soil and into the outer cortical cells of the root.
Since CePO4 is insoluble, it remains on the surface of the root which has been confirmed from TEM/EDS results.
It has two sharp blades that cut weeds from their roots just under the surface of the soil on both the push and pull stroke.
Is the surface of the compost a dense mass of fine roots?
JE are receded to the root and formed attachments on the cementum surface of the root.
Briefly, freshly extracted teeth were repeatedly rinsed with sterile phosphate-buffered saline (PBS; Corning, New York, USA), and PDL tissue was scraped from the middle of the root surface.
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