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The aligned scaffold region and 200 bp upstream from the start of the alignment were extracted.
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The following parameters were considered (see explanatory Figure 3): i) ratio of alignment length to oligo length (percent alignment); ii) maximum number of mismatches; and iii) distance from the start of oligo/transcript alignment to oligo 5' end.
As some of Jean's intimates know, she was worried sick, right from the start, that the alignment of political forces was such that "Karmel," despite having the right impulse, was constructing the wrong machinery.
Only the best alignment of each query sequence was chosen, and alignments were clipped from both ends to the start of the first match and the end of the last match positions.
From the start of his tenure, Pres.
Our alignment agreed well with the time resolved data from CLEM study: the start of Abp1 assembly coincides with initial membrane bending, and the assembly of Rvs167 starts when the invaginations are ∼50 nm long (Kukulski et al., 2012).
The diagnosis of the existing road site necessarily starts from the reconstruction of the road alignment.
Thus, the start and stop of the alignment and the sizes deduced from electrophoretic motility were used to define the ends of each sra gene.
Time zero indicates alignment to the start of stimulus presentation.
To infer these parameters from the data, we use a simple iterative approach [ 28]: Starting with an initial alignment, parameters are estimated so that the likelihood of the alignment is maximised.
Alignments not starting from the beginning of the CDS were regarded as suspicious.
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