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CAT activity from the stably integrated MMTV-CAT reporter was about 35% less in non-transfected (NT) cells treated with Dex+iAs compared to Dex alone (Fig. 6A).

Pooled colonies were assayed for the presence of the correctly sized episomal insert using primers that flanked the cloning site and genomic DNA prepared from the stably transfected cells.

In vivo, however, SET, but not hNAP1, significantly enhances transcription from the stably integrated HTLV-1 promoter/reporter construct.

In order to identify c-FOS target genes that could be involved in changing the adhesive properties of OvCa cells, we performed cDNA arrays with mRNA isolated from the stably transfected OvCa cell lines.

Media was collected from the stably transfected cells and the His-tagged antibodies were isolated and purified using Ni-NTA agarose (R901-15, Invitrogen) according to the manufacturer's recommended protocol.

For further validation of reference genes in Vanilla, two frequently used housekeeping genes, Actin1 and ubiquitin (Ubi), were selected from the stably expressed genes list, and their expression levels were evaluated in early stage seeds and in stem by quantitative real-time RT-PCR.

To do this, we selected 3 independent stable clones displaying different levels of V5-fusion protein expression from each of the stably transfected SK-MEL-28 cell lines (Figure S2).

Further, immunoprecipitates with anti-MDC1 from nuclear extracts of the stably transfected HEK293 cells contain TonEBP/OREBP-V5 (Fig. 2B).

pEPito-hCMV/SM22-EGFP-IRES-BSD could only be rescued from genomic DNA of the stably selected TE-671 cells, indicating that this construct was maintained here as an epsiome.

Based on the inspection of the standard deviations (SD), genes can be ranked from the most stably expressed (ffh), exhibiting the lowest SD, to the least stably expressed (glnA), exhibiting the highest SD.

Red cells were lysed with a lysis buffer (0.83% ammonium chloride) and 5×106 BM cells were derived after culture in 10% FBS containing RPMI, supplemented with 15% of supernatant from the GMCSF stably transfected CHO cell line [38].