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Leaf samples from the seedlings were collected to test the hybrid status of the plants.
(b) Hypocotyl growth from the seedlings shown in (a) was measured.
(d) Hypocotyl length from the seedlings shown in (c) was measured.
The higher regeneration and transformation rates were obtained from the seedlings cultured for 25 days in darkness followed by 10 days under light.
For DNA extraction and molecular analyses, leaves were collected from the seedlings after 6 months (there were from 46 to 49 seedlings per mother tree).
The coding sequence (CDS) of APIP12 was further cloned by RT-PCR from the seedlings of Nipponbare (NPB) and determined by sequencing.
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However, from the seedling establishment point of view, seed mass (i.e. reserves accumulation) best reflects the resources available for seedling (Leishman et al. 2000).
Each tree in our model is associated with its immediate circular ecological neighbourhood and is treated individually from the seedling stage through to its decay.
The primary roots of the mutant showed no gravitropic response from the seedling stage on, whereas the gravitropic response of the shoots was normal.
From the seedling stage until the stem elongation stage, the leaves and shoot phenotypes of the knockout mutant appeared wild type (Fig. 6a).
These results suggest that the primary roots of the mutant lack a gravitropic response from the seedling stage on, whereas the shoots show normal gravitropic response.
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