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Apart from the second outbreak, all the epidemics were severe, with a very high mortality rate.
§Chicken isolates from the second outbreak.
†Human isolates from the second outbreak.
These 7 isolates obtained from the second outbreak were closely related, appeared clonal in origin.
Thus, the only difference between isolates from the 2 outbreaks is a mutation (T42) in isolates from the second outbreak.
Enhanced pathogenic potential of all avian and human influenza virus isolates from the second outbreak was shown by mutation E627K in the PB2 region.
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All the above probabilities are based on a prior estimate (c) of 0.5 which assumes an equal likelihood that the second outbreak came from the first and from the background population.
Similarly, 12 samples were collected in the second outbreak from phacomachines and different parts of the operating room.
Analysis of sequences from strains detected in during the second outbreak November and December 2008 was limited to 4 strains (6510 08, 6546 08, 6547 08, 6660 08) and to the S segment (627 nt).
In the reported estimates, we assumed that c = 0.5, implying that it is equally likely that 1) the second outbreak came from the population and 2) the second outbreak came from the first case.
The second outbreak was reported from backyard poultry in Manipur, a northeast state in July 2007 [9].
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