Sentence examples for from the same host as the from inspiring English sources
The non-specific staining was blocked with PBS supplemented with 0.1% Triton X-100 and 5% serum from the same host as the secondary antibody used.
Control procedures were performed according to the same experimental protocol by (i) omission of the primary antibody, and (ii) substitution of the primary antibody with a non-specific IgG from the same host as the primary antibody (Santa Cruz Biotechnology).
Air-dried cryosections were briefly fixed in 4% PFA before being permeabilised with 1% Triton X-100 for 30 min, and subsequently blocked for 1 h with 5% serum from the same host as the secondary antibodies used, before 1 h incubation at RT with primary antibodies.
To avoid background from secondary antibodies alone, we normally pre-blocked the tissue with 5% normal serum from the same host species as the labeled secondary antibody.
When primary and secondary antibodies are used, it is most effective to block tissues by using normal serum from the same host species as the labeled secondary antibody.
As a formal test of whether genetic differentiation-after geographic distance was accounted for-was stronger among samples from different hosts than among samples from the same host, we used partial Mantel tests [ 74] as implemented in Arlequin.
It was also found that isolates obtained from the same host in different locations may belong to the same subtype.
However, not all Anicetus species from the same host were found to cluster in the phylogenetic tree: A. aligarhensis and A. dodonia, from two different hosts, appeared to cluster together as sister species with a high posterior probability value.
As there are no known in vivo fitness values of viruses from the same host, we resorted to simulations to assess the goodness of our fitness landscape estimates.
Thelytokous Lfa morphotypes from A. ruborum were clearly differentiated from Lfa morphotypes from other hosts, but less differentiated from Lco morphotypes from the same host.
