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In fact, they came from the same heart: all 30 were written, under different names, by Ms Helen Griffiths, a marketing manager from Salford.
It still comes from the same heart.
In addition, several strains can be tested simultaneously with valve segments from the same heart, whereas the surgical procedures and biological variability in the rabbit endocarditis model are complex and limit the number of strains that can feasibly be tested.
Each experiment was performed in triplicate (myocytes from the same heart).
The volumes, established by histology, were considerably smaller than those of the MRI data from the same heart.
Multiple measurements of myofilament performance from the same heart were averaged to provide a single measure for that heart.
Similar(49)
In the acyl-RAC pull-downs from the same hearts, however, only 17 and 23% of PLM is PEGylated.
RNA was extracted from FACS-sorted GFP+ and GFP− myocyte-depleted cardiac cells (20,000 cells per condition/heart, obtained from the same hearts) and from isolated cardiomyocytes using RNeasy Micro Kit (Qiagen).
Samples of coronary effluent were obtained from the same hearts after the first equilibration phase and again at the end of the second equilibration phase, each time for 3 minutes, and were stored at -20°C.
In order to verify whether the concentration of forskolin being used was effective in activating PKA-dependent pathways, we tested the effect of 10 μM forskolin on the L-type Ca2+ current (ICa,L) in rabbit atrial cells isolated from the same hearts; forskolin increased ICa,L by 2.57 ± 0.71 fold (n = 8, data not shown).
Analysis of individual optical sections collected in the short axis reveals a close correlation between areas of infarct identified by fluorescence emission after excitation at 470 nm with those identified subsequently in sections from the same hearts by histological staining with Masson's trichrome.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com