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Following fixation correlative confocal images from the same field of view show (b) microglia labelled with Iba1, (c) hippocampal neurons labelled with β-tubulin and (d) a merged image of Iba1, β-tubulin, DAPI labelled cells overlaid with the ptychographic phase image.
For QPM measurement, an off-axis interferogram is captured from the same field of view.
Bright field (visible) and fluorescence images were collected from the same field of view and correlated.
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Vessel recruitment was determined as the change in vessel number from baseline during each treatment for the same field of view.
(b-2) Confocal fluorescence image of the same field-of-view as in (a-2).
All images are saved digitally using Leica's IM500 Image Manager Database software from the same field-of-view.
An NLOM image of the collagen matrix, shown in Fig. 3F, from the same field-of-view was acquired simultaneously using SHG.
To test this, we compared the dynamics of Fluo-4 fluorescence from the same field-of-view measured either by standard one-photon or TF patterned illumination (Fig. 3).
Augmented fluorescence images (AFIs) were generated by combining the information related to cell position as given by PCM segmentation with intensity values from a fluorescence image of the same field of view.
Prior to DCE MRI, spin-echo images were acquired from the same slices and with the same field of view and spatial resolution as the time course data to provide baseline T1 data.
We defined an infected neuron as 30% brighter than background fluorescence from an area outside of the ganglia in the same field of view.
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