Sentence examples for from the reverse transcriptase from inspiring English sources
An E2DISP scaffold displaying pep23, a 15-residue B- and T-helper epitope from the reverse transcriptase of HIV-1, was able to induce a pep23-specific T-helper response in cell lines in vitro.
As shown in Fig. 2A, filtration could decrease the miRNA contamination in AMV solutions: all the CT values of four miRNAs were decreased about 1 2 cycles, which indicates that the miRNAs in AMV solution might be separated from the reverse transcriptase in this condition.
One microliter (1 μl) of cDNA from the reverse transcriptase reaction was used as the template for quantitative real-time PCR reaction with a final PCR reaction volume of 25 μl, with the 5' and 3' gene specific PCR primer concentrations at 100 nM each.
These 5′ added guanine residues are known to originate from the reverse transcriptase step in preparing the cDNAs (Carninci et al., 2006).
Three pooled RNA aliquots were omitted from the reverse transcriptase reaction to ensure the absence of products other than those originated from the reverse-transcribed mRNAs.
This is probably what occurred with TERT, the telomerase of eukaryotes, which probably originated from the reverse transcriptase (RT) of a non-LTR retrotransposon (Lingner et al., 1997).
The amount and purity of the total RNA were determined by spectrophotometer readings at 260 280 nm, and equivalent amounts of RNA from each treatment were used for the reverse transcriptase reaction using the Superscript III enzyme (Invitrogen).
No PCR products were amplified from control oocyte cDNA preparations from which the reverse transcriptase had been omitted, indicating that the PCR products were amplified from cDNA (Fig. 2).
To examine LINE-1 relationships, sequences from the 3' region of the reverse transcriptase from 21 species (representing 13 orders across Afrotheria, Xenarthra, Supraprimates and Laurasiatheria) were obtained from whole genome sequence assemblies, or by PCR with degenerate primers.
First-strand cDNA was generated from mRNA and the reverse transcriptase reaction products were used for quantitative real-time PCR, which was performed with the MyiQ Single-Colour Real-Time Detection System for quantification with Sybr Green and melting curve analysis (Bio-Rad) as previously described[30].
Complementary DNA was generated from RNA using the reverse transcriptase RevertAid™ (Fermentas).
