Sentence examples for from the reverse library from inspiring English sources
Blast2go analysis revealed that 91 ESTs, 31 from the forward library and 60 from the reverse library, displayed significant sequence homology to the genes with known or putative functions.
About 1200 gene fragments were screened from the forward library and 770 from the reverse library.
The sequencing of 200 clones from the forward (individuals incubated at 10°C versus individuals exposed at 20°C) SSH library, as well as from the reverse library (individuals incubated at 20°C versus individuals exposed at 10°C) allowed the identification of 83 different gene sequences for P. pandorae (20% of the whole sequencing effort: the other sequences are redundant).
The sequencing of 200 clones from the forward (individuals incubated at 10°C versus individuals exposed at 20°C) SSH library, as well as from the reverse library (individuals incubated at 20°C versus individuals exposed at 10°C), allowed the identification of 78 unique sequences for B. thermophilus (19% of the whole sequencing effort: other sequences are redundant).
From the four plating done for each SSH cDNA library, 492 recombinant colonies for the forward and 502 colonies for the reverse library could be picked-up.
We isolated a total of 114 subtracted clones from the forward libraries (Table 2) and 133 from the reverse libraries (Table 3).
As shown in Figure 4, the reverse library contained all functional classifications from forward one except for rubber biosynthesis (RB).
We sequenced a total of 5980 expressed sequence tags (ESTs): 1524 from the liver forward library, 1451 from the head kidney forward library, 1419 from the skeletal muscle forward library, and 1586 from the liver reverse library.
ESTs from the liver reverse library were assembled separately.
The ESTs from the 3 forward SSH libraries represented 958 (head kidney: 212 contigs and 746 singletons), 812 (liver: 200 contigs and 612 singletons) and 642 (skeletal muscle: 159 contigs and 483 singletons) putative transcripts, and ESTs from the liver reverse library represented 846 putative transcripts (178 contigs and 668 singletons) (Table 1).
Among the 2887 high quality sequences 1168 were derived from the forward subtractive library whereas 1719 from the reverse subtractive library (Table 1).
