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For qRT-PCR, 1 g wood tissue from the respective samples was used.
Results of the blanks have been subtracted from the respective samples.
Genetic sequences obtained from the respective samples were used in the phylogenetic analysis to identify the LCMV strain and epidemiologic link leading to transplant-associated deaths.
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Known amounts of Vela or Imig proteins were used as standards for the respective samples from injected mice.
Importantly, because of some differences in reactivity, Vela or Imig were used as antigen controls for the respective samples from mice.
One possible explanation for this could be related to the tissue from which the respective samples originated.
The basic description (gender and age) of the patients from whom the respective samples were taken is given in the Table 1.
Procrustes variances were obtained by summing the squared deviations from the respective sample means and dividing by the appropriate degrees of freedom.
Finally, these putative diagnostic positions from different regions of the mtDNA were genotyped in samples that showed fewer than three control region substitution differences from the respective sample that was completely sequenced (Tables S1 and Table S4).
The process is summarized briefly as follows: 3 μg of DNA from the respective sample were fragmented by a five minute nebulization at 44 psi.
Gene amplification was performed using LightCycler® 480 System (Roche), SYBR Green I Master (Roche), and a small aliquot from the respective sample sequenced by Illumina.
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CEO of Professional Science Editing for Scientists @ prosciediting.com