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To selectively precipitate the assembled minichromosomes from the reconstitution reaction we adapted a method previously used to purify short chromatin fibres [41] based on their precipitation by MgCl2 [45], an effect that can be modulated by monovalent cations [46].
Since BiFC results from the reconstitution of YFP fluorescence through the interactions of their tagged proteins, it provided us with an opportunity to study the dynamics of the U1-70K/SR45 complex by FRAP analysis.
Thus, these participants were excluded from the reconstitution procedure.
The syndrome is believed to result from the reconstitution of the flagging immune system with a resultant inflammatory reaction to previously occult and clinically asymptomatic infection.
Similar(56)
It covers the "state of the arts" with respect to the use of hemopoietic stem cells collected from the peripheral blood for the reconstitution of hematopoiesis after myeloablative therapy.
To probe the roles played by individual components in the plus-end-directed transport of dynein, we performed dropout experiments in which proteins were selectively omitted from the reconstitutions.
Their relevance to IMP reconstitution draws from the observation that the reconstitution success depends on the phase ranges explored during proteoliposome formation.
The authors describe the reconstitution from pure recombinant components and an EM reconstruction of the HIV-1 RNA export complex: i.e., a complex formed between the HIV-1 RRE and Rev, Ran GTP), and the human exportin, Crm1.
Instead, it would have to "derive from human existence, from the fundamental reconstitution of the position of people in the world, their relationships to themselves and each other, and to the universe".
He believed that "a genuine, profound and lasting change for the better... will have to derive from human existence, from the fundamental reconstitution of position of people in the world, their relationships to themselves and each other".
will have to derive from human existence, from the fundamental reconstitution of position of people in the world, their relationships to themselves and each other". This all sounds a bit New Age y, vague and impractical.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com