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Several changes in the V2 sequence from the late time point virus as compared to early time point virus were identified.
Hierarchical clustering analysis showed that samples from sham-operated animals and 24-h SAH animals clustered together and that samples from 1-h and 6-h SAH animals clustered away from samples from the late time point (24 h post-SAH).
To account for changes in the underlying distribution of confounding variables between the two time periods, ACR and eGFR from the late time period were standardized to those of the early period by indirect adjustment for the three-way categorizations of age, sex, and diabetes duration.
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Figures 5(b) and 5(c) show the normalized absorption changes obtained when the probes were placed directly on the brain (red curve with the circles) and the Δ μ a(t) recovered from the late time-window of the DTOFs when the probes were positioned on the scalp.
The similarity between the Δ μ a(t) recovered from the late time-window of the DTOFs when the probes were positioned on the scalp (blue and green curves in 5(b) and 5(c)) and Δ μ a(t) measured directly on the brain (red curve in 5(b) and 5(c)), illustrates the sensitivity of the DTOFs to changes occurring in the brain.
However, several other changes were identified in C2, C3, C4, and V4 regions from the early to the late time point virus isolate.
The values of πN/πS were lower in the participants with severe outcomes, with the lowest mean value (0.096) at the late time point from the participant with mild to severe conversion (Figure 4C).
These results indicate that the early time points from the macaque-VN1203 infection (days 1 and 2; Mac-early), the macaque-VN1203 late day time point (day 7; Mac-late), and the late time points from the Calu-3 VN1203 infection (12, 18 and 24 hour; Calu3-late) each formed separate clusters in the process term space.
In contrast, "late" virus was not neutralized by early and contemporaneous plasma samples; only plasma from the latest time point (week 181) showed low-level neutralization.
When outcomes were assessed at more than one time point, for general analyses we used data from the latest time point available, but all relevant time points were used in subgroups by study duration.
Comparisons were carried out as follows: The data were compared first from the latest time point i.e. eight hours after decrease of cerebral perfusion pressure to 45 mmHg.
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