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Particulate matter depositing during the whole smoke exposures was eluted from the inserts and quantified using fluorescence spectroscopy.
Membranes with cells were removed from the inserts and embedded in Entellan.
After removing the media from the inserts, non-migratory cells from the interior of the inserts were removed with cotton-tipped swabs.
After immunostaining, membranes were removed from the inserts with a fine, sharp, sterile razor by inserting it at one side of the filter and then gently moving it around the filter.
The bacterial plasmid backbone was removed from pTRE-Bi-SG-T using PacI and PmeI, and piiTRE-Bi-SG-Tii using AccI and NdeI, and separated from the inserts using gel purification.
The rehydration solution was carefully removed from the inserts, 0.5 ml Complete Tu (2% FBS) was added to the lower wells of the plate, and 2.5×104 cells suspended in 0.5 ml of serum-free Complete Tu media were added to each insert well.
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The eggs adopted genes from the inserted cells, and the resulting clones were grown to an early embryo stage.
These tissues, easily recovered from the insert, were then subjected to biochemical and histological analyses.
It is, therefore, possible to increase recombinant stability by removing functional motifs from the insert that may have deleterious effects on recombinant YF viral fitness.
This phenomenon can also be confirmed from the inserted maps in Fig. 7a.
The receiver obtains the phase rotations applied in the PAPR reduction from the inserted pilots.
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