Exact(10)
This was reflected from the GUS quantitative assays that showed a significantly reduced GUS staining level (to 27%-55%) in different tissues of pSH4-GUS transgenic plants (Figure 5).
The isolated fragment of potential promoter in the vector pMD18-pSH4 was inserted into the XbaI/BglII sites of vector pCAMBIA1301 to replace the CaMV35S promoter upstream from the GUS reporter gene to generate a recombinant vector, pSH4-GUS (Figure 6A).
Balabushka cues from the Gus Szamboti era are typified by straight grained maple forearms bearing four ebony points, with four veneers included, normally colored black, green, white and mahogany, or sometimes with an orange veneer in place of mahogany.
In contrast to dcl4 enhancement of secondary siRNA accumulation, the dcl2 mutation eliminated secondary siRNAs including those (probe 2) that could be derived only from the GUS sense transgene mRNA (Figure 3B, lanes 6 9, probes 2 and 3).
In progeny of crosses with the 6b4/306 line, both P1/HC-Pro and P38 restored accumulation of full-length mRNA from the GUS sense transgene and eliminated secondary siRNAs (probes 2 and 3) (Figure 4B, lanes 3 8).
In the 6b4/306 background, the dcl4 mutation eliminated accumulation of loop mRNA and greatly increased accumulation of secondary siRNAs including those (probe 2) that could be derived only from the GUS sense transgene transcript providing additional evidence that impairing DCL4 activity promotes a shift to transitive silencing (Figure 3B, lanes 11 14, probes 2 and 3).
Similar(50)
After the GUS staining, Arabidopsis seedlings were treated with 70% ethanol to remove chlorophyll from the GUS-stained tissue.
As could be seen, the signal from the GUS-gene was present in all samples (row L).
The clone was sequenced and the NcoI- SmaI fragment that included EDS5H 3xcMyc-taggg was thEDS5H 3xcMyc-taggMBIA 1304 (was.CAMBIA.org) from which thenGUS and GFP genes had been removed.
The 4XPOS9BC element was combined with the 35S minimal promoter from pLAW105 and the GUS coding region from pCGN43 and inserted into pMON999 [ 11] as a PstI/StuI fragment adjacent to the nopaline synthase polyadenylation region.
The CaMV 35S minimal promoter (-46 to +7) fused to the GUS gene from pBT-GUS [ 45] was cloned as a BamHI and XmaI fragment into pPAM (GenBank AY027531).
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