From the emergence of the first leaf after germination through to flowering, each wild-type leaf adopts a unique identity based on size and shape, and this graded growth variation is often referred to as the "leaf heteroblastic series" [ 23].
Second, the authors used the third leaf (from bottom to top) for maize, while for sugarcane we used the first leaf (from top to bottom) with the dewlap fully exposed.> -wrap-foot> Valueshownwn are mean ± SD (n = 5) and referred to μmol g-1 FW, except for myo-inositol (nmol g-1 FW).
Root, root bark, stem, petiole (the petiole of the first young leaf), young leaves (the first leaf from the apex of the side branches) and old leaves (the fifth leaf from the apex of the side branches) were prepared from the same tree for expression analysis as described previously [ 52].
Young leaves (the first leaf from the apex of side branches, including the apex) for library construction and gene cloning [ 52] were collected from a C. acuminata tree with a diameter of 14 cm cultivated in the greenhouse of the IMPLAD Institute of Medicinal Plant Developmentt), Beijing, China.
Ultimately, both shoot and root of the sporophyte grow out from the gametophyte, but, even after the first leaf has begun to photosynthesize and thus to produce its own food, the gametophyte may persist.
The second leaf from each seedling was removed and the first leaf attached horizontally, adaxial side up, on a Plexiglas sheet with plasticine (Ulmann, Paris, France) [ 60].
Four readings from the seventh leaf (Cob Leaf) from the six tagged plants per experimental unit (plot) were taken.
In the BC4F4 generation derived from a cross between IR24 and IRGC105715, one of the ILs showed the hairy-leaf trait from the sixth leaf to the flag leaf, and was named IL-hairy (Fig. 1c, d).
From the second leaf stage to the time of rooting assessment, the water level was maintained at the level of the soil surface.
