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The dendrograms were visualised from the files generated by the ClustalX alignment using MEGA version 3.1 [ 159].

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Divergence between D. melanogaster and D. simulans were parsed from chain files generated by the University of California Santa Cruz Genome Browser (genome.ucsc.edu).ucsc.edu

Bases are filtered for a minimum quality score of 30 and a minimum read depth of 15x from VCF files generated by the Genome Analysis Toolkit [ 65].

Sequencing reads were extracted from the image files generated by the Illumina/Solexa 1G Genome Analyzer.

In the fasta files extracted from the gff files generated by the EuGene pipeline, genes were tagged as "Truncated" if they lacked start or/and stop codons.

Read sequences were called from the chromatogram files generated by the ABI3730xl using Phred (Ewing and Green 1998; Ewing et al. 1998).

Each XML BCP was converted into a list object in R. Its abundance measurement was parsed from the data files generated by the IbisTrack software and stored thereto.

Our method uses the BAM files that are generated by BWA [ 24], which aligns the FASTQ-formatted read pair files generated by the sequencer from the donor genome's (i.e. the genome under interrogation) DNA.

ImageJ from the NIH (http://rsbweb.nih.gov/ij/) was used to convert the ZEN format files generated by the Zeiss software into TIFF files recognized by the CellProfiler software.

The.RAW files generated by the LTQ-Orbitrap XL were converted to.MSM file format peaklists using "Quant" module from Max Quants (v 1.0.13.13, Max-Planck Institute for Mass Spectrometry, Martinsried, Germany [ 13, 14].

Observed and predicted responses were taken from the SAMPLS.out file generated by the SAMPLS program.

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