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This indicates that the RANKL-induced osteoclast differentiation from the differentiated PBMC/osteoblast co-culture system was significantly inhibited by vitamin D3.
(b) Which sets of genes enable the best distinction of the pluripotent stem cell state from the differentiated one, considering their (differential) expression?
However, with regard to the differentiation potential, the pluripotent cells of embryonic origins completely repress the expression of differentiation-associated genes, while iPSCs derived from the differentiated cells may inevitably retain an imprint from their origins despite being reprogrammed to a fully pluripotent state.
However, it is crucial to remove residual undifferentiated hESC from the differentiated population to avoid teratoma formation in vivo.
ALP levels in 0.75 mM and 1 mM GO-treated cells were significantly different from the differentiated control as calculated by paired t-test (n = 10, * = p < 0.01).
The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation.
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Hierarchical clustering demonstrated that naive cells were distinct from all the differentiated subtypes.
However, the proportion of oligodendrocytes differentiating from hPSCs among the differentiated progeny is generally very low [ 17].
Culture medium from SSCs and the differentiated cells was collected over 2 days from equivalent numbers of cells.
This enables distinction of the least differentiated gonocytes (OCT4+/VASA−) from the most differentiated spermatogonia (OCT4−/VASA+) with some germ cells intermediate (OCT4+/VASA+).
On encountering antigen CD8+ T cells differentiate from the least differentiated (naïve or early memory) stage to the most mature (memory/effector) stage.
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