Sentence examples for from the clarifying from inspiring English sources

— is a frequent area of disagreement, though, and one that benefits from the clarifying efforts of social work and jurisprudence.

Working from this principle to specifics who's vulnerable, and when?—is a frequent area of disagreement, though, and one that benefits from the clarifying efforts of social work and jurisprudence.

As flow-through devices, it is then possible to collect a concentrate of particulates by drawing off the particle stream and separating it from the clarified fluid and so can operate continuously as opposed to batch processes such as centrifugation.

GST-PTB was then purified from the clarified lysate using glutathione-sepharose, washing with PBS containing 0.5 mM DTT, 10 mM EDTA and 1% Triton X-100.

hLSD1 was purified from the clarified lysate with Ni-NTA agarose (Qiagen) and further purified by anion exchange chromatography (MonoQ column (Pharmacia Biotech)) and gel filtration (Superdex 200 (Pharmacia Biotech)) using standard protocols.

The protein was purified from the clarified cell lysate by immobilized metal affinity chromatography binding on Ni Sepharose High Performance resin (GE Biosciences, Piscataway, NJ) equilibrated with Binding Buffer (20 mM HEPES, pH 7.2 7.4, 300 mM NaCl, 5% glycerol, 30 mM Imidazole).

Supernatants from the clarified samples were used for isolation of RSV by CEC [5] and presence of RSV was detected by indirect immunofluorescence staining with a blend of monoclonal antibodies that detects both group A and group B RSV (Chemicon International).

HSV-2 was pelleted from the clarified supernatant by centrifugation at 20,000 g × 2 h.

GST-tagged proteins were purified from the clarified lysates using Sepharose 4B GST affinity resin (GE Healthcare) according to the manufacturer's instructions.

GST fusion proteins were then isolated from the clarified bacterial extract by incubation (1 h, 4 °C) using glutathione Sepharose beads (GE Healthcare).

After the cell pellets had been solubilized, the supernatant fraction from the clarified lysate was incubated with Protein A/G UltraLink (Pierce, catalog no. 53132) overnight while being shaken at 4 °C.