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The obvious straight microtubules mainly existed in the range of 0 2 μm from the bottom of the cell, while the microtubules mostly intersected with each other in the upper part of the cell.
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Interestingly, we found that viruses moved unidirectionally in the range of 0 2 μm from the bottom of the cells.
Sheets of such a material could be laid down on the bottom of the cell, separated from the cell itself by an electrically insulating layer.
"You can knock it off its hinge and then slide out the bottom of the cell.
It was shown that the symmetry breaking is based on mRNA migrating from the bottom of the yolk cell upwards.
When the cells were detached from the bottom of the well, the cells were resuspended in 1.2 ml of culture medium to neutralise the trypsin.
After incubation, the magnet was removed and the cells were detached from the bottom of the plates, forming a contiguous cell sheet.
The cytotoxicity effect was characterized by aggregation of degenerated cell clusters detaching from the bottom of the wells forming holes (gap formation) within the cell monolayer.
After the incubation period, a sterile cell scraper was used to gently detach the cells from the bottom of the plate.
When the outgrowth of the epicardial cells was confluent, the cells were detached from the bottom of the culture dish with trypsin/EDTA (Invitrogen) solution.
The NGF-attracted cells were trypsinized from the bottom of the Transwell insert and cultured in growth medium.
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