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Brightfield microscopy was used to assess nanoparticle diffusion from the bead.
where K is a specified force constant which is given to 5.0 kcal/(moleÅ2), r is the distance from the bead to the center of the sphere, and R is the radius of the sphere.
One day they observed a curious phenomenon: Sometimes a bead became "tethered" to the bottom of the dish, apparently by a single strand of plastic that had frayed from the bead.
Further, the beads exert force on the surrounding collagen gel and cells, resulting in intratissue strains of 0 10% tunable with bead dimensions, collagen density, and distance from the bead.
DNA methylation values from the bead array hybridizations were scored as β-values [16].
To obtain concentration values, mean fluorescence intensities from the bead combinations were analyzed by the MasterPlex QT quantification software.
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To enumerate the encapsulated probiotic bacteria, at first the captured bacteria were released from the beads.
Thus, formation of an alternative hybrid between the competimer and the template DNA during bead hybridization displaced unligated RCOs from the bead-bound species and resulted in an estimated 10-fold increased sensitivity.
Two sets of oligonucleotides were used to disrupt these structures; one to displace unligated reporter labels from the bead-bound species and another to occupy sequences which interfered with array hybridization.
We verified however that imputation gave almost identical values to the alternative procedure of recalculating β-values from the bead-replicate averaged U and M values, thus validating both imputation approaches.
Plasmids were subsequently eluted from the beads by IPTG.
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