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In the immobilized cells from the assays with and without pH control no visible changes were detected on the microscope.
The signal strengths were not significantly different from the assays at 4°C.
In addition, we found that use of either sonication, pestle treatment, or EDTA (known to disperse Asc10-mediated bacterial clumping [20]) for removal and resuspension of the bacteria attached to the valves resulted in similar numbers of CFU on the enumeration plates from the assays carried out with Asc10+ strains (see Supporting Information: Figure S1).
In reference experiments without PMO the background signal (H2O2 production by CDH) was measured and subtracted from the assays.
All exonuclease assays were performed under steady-state conditions as described above except that dNTPs were omitted from the assays.
Therefore genomic localization and biological roles of other reliable but less reactive TF-binding loci (low avidity BSs) cannot be identified from the assays [ 9, 11, 12, 14].
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Mortalities were recorded daily for 7 days and were only considered infected if V. parahaemolyticus was recovered from the assayed fish.
The data obtained from the qPCR assays closely matched the data obtained from the microarray experiment.
Intriguingly, the EGF-induced signal from the pHER2 assays trended with that of the pHER1-HER2 assay.
The findings from the DPPH assays correlated well with IC50 values derived in ABTS experiments.
Absorbance values from the Tube assays parallel but do not equal those from PTA-ELISA tests.
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