For probe construction, total RNA from the acclimatized colonies was extracted using Trizol (Invitrogen) and an RNeasy Mini Kit (QIAGEN), following the manufacture's instructions.
To measure gene expression variation from the acclimatized colonies in the common garden, a cDNA microarray platform comprised of 8,386 unigenes developed in collaboration by two coral genomics research groups from the Australian National University and James Cook University [ 40] was used.
Despina Stamataki, the PhD student from Crete, having acclimatized to the weather, collaborated with Johan Ericson (now back in Stockholm) and his lab to test the involvement of Gli activity and the function of Gli3 in neural patterning.
In particular, PFK was greater in heart and skeletal muscle from individuals acclimatized to the low oxygen swamp site, while CCO was lower in heart after hypoxic acclimatization.
We had grown used to the language, acclimatized to the name-calling, listening until we could distinguish the venomous from the merely misguided.
16 Once the mice were acclimatized, the rod was progressively accelerated from 4 rpm to 40 rpm over a duration of 5 minutes.
If cardiomyocytes from fish acclimatized at 14°C were exposed to 7°C, a significant decrease in I(Ca2+) was measured, but if Epi were added, I(Ca2+) increased by a factor of 7.2, indicating a significant increase in adrenergic sensitivity in response to cooling [ 12].
The alga was isolated from temple tank, acclimatized and successfully mass cultivated in open raceway pond at semi-continuous mode.
Female NOD mice (4 weeks old) were purchased from the Laboratory Animal Resource Center of the Peking University Medical Science Center and were acclimatized to the center for 1 week before starting the experimental protocol.
Tolerant bacterial isolates were collected from five different acclimatized activated sludge batch reactors treated by synthetic wastewater (Table 1) with elevated levels of Cu, Cd, Co, Cr (prepared as 10%% stock solutions of CuSO4, CdCl2·H2O, K2Cr2O7, CoCl2·6H2O salts) and metal mixture (El. Bestawy et al. 2012) as described by Özbelge et al. (2005).
Seventy-two female Tg2576 mice, over-expressing the human APP695 (Swedish) mutation, on a B6; SJL mixed background (strain #1349) (Hsiao et al., 1996) aged 10 12 weeks were purchased from Taconic and acclimatized until 26 weeks of age.
