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To track regeneration on whole-body level over time, we developed a morphological scoring system composed of an additive body score summed up from single tissue scores at distinct time points.
A beginning outgrowth of an adherent cell layer from single tissue pieces was observed after approx. 10 days.
This represents a powerful technique: it does not require orientation, it can be carried out on complex glandular structures and is not affected by the biases involved in measuring labelling indices from single tissue sections.
In fact, performing such examination on miRNAs sampled from single tissue, organ, development stage or cell line might cause bias because the miRNA's expression profile in different tissues or stages are usually distinct.
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Thus, analysis of tumour genome data from single tissues results in low recall characteristics that are qualitatively similar to those observed earlier in the aggregate analysis.
Numerous approaches have been applied to co-expression maps to infer gene function information from single tissues, entire organisms or across species [ 13, 14], but they have also been employed to determine the differences and similarities between species [ 15, 16].
Estimates of the frequency of mosaicism range from 67%to90%0% (Held et al. 1992; Fernández-García et al. 2000), but the presence and degree of mosaicism has been difficult to establish because multiple tissues must be studied and PCR-based methods must be used for accurate quantification, but most studies have relied on karyotype data from single tissues (Fernández-García et al. 2000).
Instead, others cDNA libraries constructed from single tissues (DiG01, DiG02, Hae00, Hae05) or from mussels treated with organic compounds and heavy metals (GDG01) or heat-inactivated bacteria (Gll01, Hae01, Hae02, Hae03, Hae04) have been sequenced to a less extent because of the more committed nature of their transcriptome.
In conclusion, reproducibility of (R -[11C]verapamil PET studies was best foR -[11C]verapamilm single-tissue (6%) and constrained two-tissue (9%) comPETtment modelstudies
Hereafter we refer to the replicated expression data from a single tissue from one species or one type of hybrid (either HXLXB or HXLXM) as a "treatment".
We show that it is possible to reliably identify eQTLs across many different tissues and also to obtain tissue-specific eQTLs by combining samples from a single tissue derived from many different experiments.
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