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Grain-cluster is formed via numerous twinning operations starting from single nucleus, and the process is referred to as multiple-twinning.
To minimize the DNA loss and maximize the yield of the extremely small amount of transposed DNA (<0.1 pg) from single nucleus, we added a large amount of plasmid DNA (30 ng) as a carrier DNA together with 3 μl of RLT Plus buffer (QIAGEN) to the mixture immediately after the stop step.
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Grindberg, R. V. et al. RNA-sequencing from single nuclei.
Grindberg, R.V. et al. RNA-sequencing from single nuclei.
Image analysis of DNA content was performed from single nuclei of melanoma monolayer imprints made from fresh frozen tissue of 14 patients with primary malignant melanoma and 16 patients with local recurrences at the incision site and local or distant metastases.
For the analysis, only signals from single nuclei were considered (10 nuclei assay−1).
Electron backscattered diffraction measurements revealed that bands and lamellae of a solid phase are continuous and originate from a single nucleus.
(A ) A low magnification image of a section from a single nucleus with two RNs (outlined with red dashed box).
In retrospect we should have chosen a better image that was able to be cropped and displayed as information from a single nucleus.
Here, the numerous daughter nuclei generated from a single nucleus without detected DNA synthesis (McEnery and Lee 1976) imply that the Agamont I nucleus already contained many genome copies.
The term "stack" is used to describe a region in which resonances arising from nonequivalent nuclei are coincident and multiplet, m, to describe a resonance arising from a single nucleus (or equivalent nuclei) but where coupling constants cannot be readily assigned.
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