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It's even more reproducible, and we can get more data from single cells.
We wanted to progress to whole-genome sequencing from single cells.
Picelli, S. et al. Full-length RNA-seq from single cells using Smart-seq2.
Zhang, K. et al. Sequencing genomes from single cells by polymerase cloning.
Individual fit from single cells were pooled together and values are expressed as mean ± SEM.
Macaulay, I. C., Ponting, C. P. & Voet, T. Single-cell multiomics: multiple measurements from single cells.
b, Two example distance maps from single cells for each embryonic segment as indicated.
Krekelberg, B., Boynton, G. M. & van Wezel, R. J. Adaptation: from single cells to BOLD signals.
Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving.
So here they're actually invasive recordings, where they record either from single cells or groups of cells at a time.
Fluorescence information from single cells was collected with a microscope objective and focused to a photomultiplier tube (Hammamatsu).
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