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We isolated Rivulus marmoratus mitochondrial DNA by long-polymerase chain reaction with conserved primers, and sequenced it with 36 sets of internal conserved primers, which were designed from the extensive sequence similarities of mitochondrial DNA from several fish species.
Anisakis simplex L3 was isolated from the intermediary host Micromesistius poutassou (blue whiting) purchased from several fish markets in Zaragoza.
Investigation of other non-infected trout from several fish farms in the United Kingdom showed similar activity in their sera towards SpSsp1.
The novelty of this platform is that it used highly conserved probes from several fish species, permitting application of the array to studies involving turbot and zebrafish.
On the other hand, exceptions with syntenic location or direct co-localization of both rDNA clusters (or their linkage to other multigene families) has already been documented in a variety of vertebrates [ 109, 110], including reports from several fish groups [ 111] as well as in loaches of the family Cobitidae [ 98], a sister lineage to nemacheilids.
After 96 h exposure, individual blood samples were taken from several fish in control (n = 12), 5 mg·L−1 (n = 12) and 15 mg·L−1 (n = 12) of atrazine groups and from all fish in 20 mg·L−1 (n = 10) a 30 mg·L−1 (n = 4) of atrazine experimental groups.
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Analysis of the syntenic regions from several fishes strongly suggests that the smelt acquired the AFP gene from the herring.
Recently, fucose-specific lectins, F-type lectins, have been isolated from the serum from several fishes such as Anguilla japonica [ 30], Anguilla anguilla [ 31], Morone saxatilis [ 32], Sparus aurata [ 33], and Dicentrarchus labrax [ 34].
Consensus primers were designed in conserved motifs and used to isolate full length cDNA from livers of several fish species by Rapid Amplification of cDNA Ends (RACE).
In this Review, we discuss our current understanding of NAFLD (particularly NASH) pathogenesis as deduced from work with several fish models of this disease.
Samples from liver, spleen, kidney and external lesions of several fish from marine cage farm and inshore fish farm were cultured on alkaline peptone water (1% NaCl, pH 8.6) and incubated at 37°C for 18 to 24 h.
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