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Since the size of the cell population was considerably large, images from separate fields were acquired and analysed as statistical samples of the whole population.
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Captured images were analysed using AxioSet software (Carl Zeiss Ltd ,UK) to calculate the mean intensities of the Texas Red and DAPI signals from ten separate fields taken from each slide.
For these cases it is shown that a combination of the velocity fields from two kinematically admissible velocity fields sometimes allows the upper bound value of surface traction to be reduced below the values calculated from the separate fields.
It follows that a collapse load for a combination of two arbitrary kinematically admissible velocity fields can be calculated as a weighted average of the two collapse loads calculated from the separate fields.
At least 3 coverslips and 200 cells from 10 separate fields were counted and scored for positive nuclear green flourescence.
To measure neurosphere sizes, images were obtained from 3 separate fields per well, and the volumes of neurospheres estimated using a microimage analysis system from Olympus.
Adipocyte size was quantified on at least 30 cells from 3 separate fields in 7 WT and 5 Cnr2 −/− HFD-fed animals, using Image J software.
Live macrophages from 12 separate fields of each chamber were counted.
Colonies from 10 separate fields were counted using a microscope with a × 4 objective.
Scoring of invading cells from four separate fields was performed for triplicate filters using the Image Pro Plus software (Media Cybernetics).
The data at each time point were derived from four separate fields, and the bars denote standard deviation.
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