Sentence examples for from reverse transcriptase from inspiring English sources

If CT value in experimental group is far below the base line CT value, miRNA contamination from reverse transcriptase may not affect the results; otherwise the possibility of false negative data should be taken into consideration because the high background might conceal the changes of miRNA levels.

FISH with a probe derived from reverse transcriptase domain of a LINE-like element, resulted in dot signals in centromeric regions on all chromosomes.

Therefore, because the sequences of coding genes in the 4.5 kb fragment are almost identical to those obtained from reverse transcriptase PCR (unpublished data), with only three substitutions in the 1372 nucleotides being compared, this first scenario is unlikely.

Furthermore, they are unlikely to be artifacts from reverse transcriptase template switching during library preparation [ 41, 42], because the breakpoints align with exon-boundaries, suggesting that the fusion event occurred prior to splicing.

Although we attempted to minimize potential artifacts from second-strand synthesis from reverse transcriptase, it is still possible that some of the observed transcription might be due to reverse transcription.

Sequences were obtained from reverse transcriptase polymerase chain reaction (RT-PCR)–amplified portions of the nucleoprotein (positions 1,157 1,477), glycoprotein (positions 4,297 4,817), and psi (positions 5,069 5,424) regions based on the reference strain M13215 (17 ).

Probes for AChE1 and the putative cytochrome P450 were synthesized from template derived from reverse transcriptase-PCR performed with the AccuScript High Fidelity RT-PCR Stratageneratagene) on total RNA isolated from tick larvae using the Totally RNA Kit (Ambion).

In order to isolate CTC from cancer patients affected by carcinoma, studies have advanced from Reverse Transcriptase-Polymerase Chain Reaction- (RT-PCR) based methods to immunomagnetic enrichment of tumor cells exploiting the presence of molecular markers as EpCAM on the CTC surface.

This avoided incorporation of misleading chimeric data that can derive from reverse-transcriptase template-switching (Roy and Irimia 2008).

The high mutation rates, resulting from reverse-transcriptase (RT) lack of proofreading activity coupled with a rapid replication rate and recombination, ensure that a number of highly related but nonidentical variants are produced.

During the MONET trial, the mean annual perpatient cost of antiretrovirals was £7780 in the triple therapy arm, of which 56% was from nucleoside reverse transcriptase inhibitors and 44% from protease inhibitors.