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We also demonstrate that B-lymphocyte clones specifically reacting to epidermal proteins exist in Castleman's tumors from PNP.
Two patients from PWP group reported increased pain in their legs and one patient from PNP group reported an unpleasant tingling sensation (i.e. paraesthesia) in their legs.
Most of the CD cases complicated with BO had suffered from PNP [ 9, 36], and the pathogenesis of complicated PNP and BO was unknown.
The amount of p-nitrophenol released from pNP esters at 30°C was monitored at 405 nm for 10 min using a spectrophotometer Synergy™ 2 (BioTek).
Similar(56)
One unit of enzyme activity is defined as the amount of enzyme that released 1 μmol of PNP per minute from PNP-α l-Ara f under these conditions.
Cell-free extracts obtained from PNP-grown culture that contained 20 μg mL−1 protein degraded 90% of 720 μM PNP within 5 h of incubation.
Enzymatic activity was quantified by measuring the p-nitrophenol released from PNP-glycoside at 400 nm wave length.
One unit (U) of DT-bgl or PF-bgl activity was defined as the amount of enzyme required to liberate 1 µmol p-nitrophenol (pNP) from pNP-β-d-glucopyranoside per min at 80 °C and pH 6.0.
aPercent activity relative to glucose or pNP released from pNP-β-D-glucoside.
β-Glucosidase activity was measured by quantifying the release of pNP (p-nitrophenol) from pNP-βGlc as described previously [ 50].
The amount of free cellobiohydrolase was quantified as pNP release from pNP-lac as described by Jalak and Väljamäe [ 16].
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