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Processed chamomile flowers first class (Fig. 1b) are obtained after cutting the stems from picked chamomile flowers.
Tan et al. (2013) proposed an inversion method based on time differences calculated from picked arrival times, which circumvented the issue of origin time inaccuracies.
DNA from picked clones was analyzed for proper targeting by southern blotting using a BglII digest as outlined in Additional file 2: Figure S2.
Liquid batch cultures were first established in BBM from picked colonies and allowed to reach the early stationary phase at 25°C and 120 rpm in a lighted shaking incubator (Innova 43, New Brunswick Scientific, Enfield, CT).
The measurements using the ToF are still valuable in that they confirm that the seen blank levels are actual U counts (from picked up ambient U) and not machine background from other scattered ion species.
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