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"To extract more detail from our specimen, we take over a hundred images from different angles, producing a block of data rather than one 2D image," he adds, explaining that the difference between this method and conventional electron microscopy "is analogous to the difference between an X-Ray and an MRI".
DNA isolated from our specimen AS35 of P. boveana from the population in Ain Shennarah (see below) was used by Genetic Marker Services (Brighton, United Kingdom; http://www.geneticmarkerservices.com) to develop a microsatellite-enriched library and to design and test microsatellite primer pairs.
However, because these two forms are distantly related, we also used the NADH dehydrogenase subunit 4 (ND4) sequences deposited in GenBank (EU363095 and EU363181) from Mulcahy [ 20] to compare to ND4 sequence (derived from the transcriptome described below) from our specimen.
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The variations in the number of lymph nodes retrieved from our specimens are, therefore, similar to other studies.
Gram-positive cocci were the predominant bacteria cultured from our specimens and Gram-negative rods were the second most common bacteria isolated.
A large amount of tissue (100 mg) was used to extract DNA from our specimens and this might explain why EBV was detected at a higher frequency than in other studies.
However, this is not true for our specimens from 4000 m, which have a pure C3 diet.
Negative controls were conducted by adding PBS solution instead of the primary antibody, and positive controls were conducted by staining known-positive samples from our pathology specimen bank.
121 pre-characterized respiratory specimens (68 culture-positive for MTB complex, 24 culture-positive for non-tuberculous mycobacteria and 29 culture-negative) taken from our frozen specimen bank were tested for the presence of MTB complex by the three assays.
Even assuming that similar mt:nuclear DNA ratios were purified from all our specimens, nuclear-genome size differences among species could account for differences in the molar quantity of mtDNA present in the processed mixed DNA sample.
Furthermore, the thallus size of G. filamentosa recorded in Chou (1945) [3.5-5 cm] is generally larger than our specimen from the Philippines, which was only 2.5 cm in height.
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