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All providers in the division used the process, but some evolved from one template to another over time.
Not surprisingly, during cDNA synthesis in vitro reverse transcriptase can also switch from one template to another and, thereby, generate artificially deleted or chimeric cDNA transcripts [ 96– 96].
In a subsequent infection cycle, RT may switch from one template (the donor) to the other (the acceptor), producing a mosaic HIV-1 genome [ 1, 2].
It is to amplify a single DNA template from minimally diluted samples and generate amplicons that are exclusively derived from one template.
Firstly, all external calibration curves are generated from one template dilution series, rather than several series required with independent calibration constructs (for example, three would be required for measurement of E2, E6 and HMBS), thereby reducing the amount of manual handling needed.
Some of the crossover sites were characteristic of classical recombination hotspots with a stretch of AU-rich sequence that promotes dissociation and switching of RNA polymerase from one template to another [40], [41].
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As shown in Figure 1, the analysis system started from only one template initialized with random values, which is called Guard template.
And third, as current NGS technologies incorporate PCR steps in their library preparations, multiple reads originating from only one template might be sequenced, thereby interfering with variant calling statistics.
However, mixed clusters can be grown starting from more than one template, in which case the base calls of the cluster cannot be accurately determined.
Their respective protein domain annotations are obtained from InterPro [ 10], from which we collect the sequences of domain instances which have at least one template domain instance from 3did.
A garland of pine cones, rescued from a blue spruce blown down a few weeks before, hung from one mantel template while a trio of variegated holly wreaths dangled from another.
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CEO of Professional Science Editing for Scientists @ prosciediting.com