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Colocalization was considered positive when two positive pixels from one stain of interest were located within a 3 pixels radius from one pixel of the other stain of interest.
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"No one stained my honour," she wrote.
Viable and non-viable diatom cells were distinguished from one another by staining with the dye Sytox Green (Molecular Probes Inc., Eugene, OR, USA) in DMSO.
In PBMC cultures, individual cell types were distinguished from one another based on differential antibody staining and on FS and SSC properties.
A large amount of information is lost when one labels a tumor as a mere ER-positive one, because a tumor in which 15% of cells exhibit weak ER staining is biologically different from one that demonstrates strong intensity staining in about 90% of cells.
All dishes from one cell line were stained at the same time point.
All experimental animals survived the experiment and contribute with data to all analyses apart from one unsuccessful ex vivo staining.
Tissues were scored for LC3A/B staining from one to four (low to high) for all resected tumor tissues.
In addition, incomplete repression results in variegation for X-gal staining from one egg chamber to another (Josse et al. 2007).
If cores from one tumour differed in staining intensity, the median score of the three related cores determined the score of the tumour.
(B) ROS production by CD8+ PBL from one SSc patient, simultaneously stained with 2 μM DCFH-DA and monoclonal antibody anti-CD8 PE for 20 minutes, was analyzed by FACS analysis.
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