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Total RNA was isolated from isolated cells using TRI Reagent (Ambion).
Potential differences between expression profiles may exist when comparing results from isolated cells with those obtained from RNA isolated directly from articular cartilage.
Tissue engineering involves the generation of new tissue from isolated cells with certain scaffolds and signaling molecules [2].
Following incubation at 0.5 and 2 h, cytotoxicity was characterized by measuring lactate dehydrogenase release from isolated cells.
Experimental models of skin include pathological conditions, phenomena such as aging and organogenesis, as in the hair follicle grown from isolated cells in vitro.
RNA was prepared from isolated cells as described above.
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The Ca++-chelators CDTA and imidazole extracted a pectin rich fraction from isolated cell walls which was however contaminated with significant amounts of hemicelluloses.
Saline-extracted cell walls were prepared from isolated cell walls of callus as described above.
DOI: http://dx.doi.org/10.7554/eLife.01355.021 Quantification of total lignin content from isolated cell walls in wild-type maize (cv.
Further to delineate the cellular origin of the observed erythropoiesis signature, we assessed its overlap with the gene-expression signatures that we derived from a publicly available dataset derived from isolated cell populations [ 19].
Therefore, to examine expression of this reporter gene in B cells, we employed magnetic bead (MACS) technology to purify the B cell populations from transgenic mouse spleen, and prepared RNA from the isolated cells.
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