The ensemble-averaged PSDF in the turbulent flame is computed from individual samples of the PSDF from large sets of Lagrangian trajectories.
Therefore, in order to ensure the validity and significance of differences in data obtained from individual samples, considerable effort was spent to ensure the reproducibility of the data generated.
The results were staggering: Not only had the animal apparently come from a known population in the Black Hills of South Dakota, it's DNA precisely matched genetic samples that had been collected from individual samples of scat — that is to say, cougar shit — or hair found in Wisconsin and Minnesota.
Two independent cDNA preparations were obtained from individual samples of the same RNA isolate.
Standard curves were generated using pooled RNA from individual samples within each experiment.
In our experiments all microarrays of pooled samples were analyzed identically with microarrays from individual samples.
Total RNA from individual samples was obtained according to the manufacturer's protocols using RNeasy Midi kits (Qiagen).
Mononuclear cells were isolated from individual samples by Ficoll (Biocoll Separating Solution, Biochrom AG, Berlin Germany) density gradient centrifugation.
Total RNA was extracted from individual samples using TRIzol® reagent (Invitrogen) and was resuspended in 20 µl DEPC-treated water.
The 8 CR microarrays consist of 6 microarrays from individual samples and 2 microarrays each produced using pooled RNA samples from 3 other individual mice.
For clonal bisulfite pyrosequencing, PCR product from individual samples was generated by non-biotinated primers (Table S2) and subsequently TA-cloned into pGEM-Teasy vector (Promega).
