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Total RNA was isolated from individual constructs using protocols described in the QIAquick® Spin gel extraction and Rneasy® kits (Qiagen, West Sussex, UK), as previously described [ 43].
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We derived the raw event rates from individual studies and constructed 2×2 tables with raw number of events and total population of the trial.
Note, in particular, that the variable sets in the rotated parts of the questionnaire included constructs formed from individual variables and that the items forming a construct were not split across the questionnaire forms.
A calibration curve was constructed from individual ant measurements.
The LOMOS-mini is low-cost, easy to construct from individual microelectronic components and has an autonomy of several months in the field.
These methods include a subject's multicompartment head model (scalp, skull, dura mater, cortex) constructed from individual magnetic resonance images, multidipole source model, and regularized linear inverse source estimates of cortical current density.
At this scale the machines are constructed from individual atoms and, according to Ralph Merkle from Zyvex, "It really does matter how atoms are arranged".
All were sequenced using "traditional" sequencing approaches in which sequencing libraries are constructed from individual segments of the genome (such as those contained within bacterial artificial chromosomes (BAC) clones) and are sequenced via gel electrophoresis and dideoxy terminator chemistry (i.e., "Sanger sequencing").
Phylogenies constructed from individual nucleotide gene regions however did not always support the groupings recovered from the concatenated dataset (Additional file 1: Figure S4).
The tunable nanopores on a quartz nanopipette platform are fabricated using the electroetching of a graphene-based membrane constructed from individual graphene nanoflakes (ø ∼30 nm).
Causal networks constructed from individual relationships curated from the literature are particularly suited for this task, since they create mechanistic hypotheses that explain the expression changes observed in datasets.
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