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Measurements of TER were also performed using a volt-ohm meter to determine the relative effect of tight junction permeability on DTPA absorption from individual cell cultures.
Measurements of TER from individual cell cultures were made using a volt-ohm meter 24 h prior to the addition of 10 μL of Tc-DTPA in PBS; one CF line (n = 12) and four non-CF lines (n = 30).
Furthermore, we have analysed the expression of miRNAs from individual cell populations from the airway and lung.
Total RNA was extracted from individual cell lines using RNeasy Mini Kit (Qiagen, Valencia, CA) and quantified using a NanoDrop 1000.
We assumed that the data from individual cell types would include genes that were directly and indirectly linked to gata3 function in those cells.
The x, y and z coordinates from individual cell tracks were obtained with Imaris.
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Thanks to me, cosmos were growing tall against the fence in our backyard, potted geraniums decorated our deck, impatiens were transferred from individual cells in plastic flats to the rich soil of my flower beds.
Likewise, in plants it is often possible to grow complete embryos from individual cells in tissue culture.
A major aim of neuroscience is to provide a description for how our thoughts and behaviour arise from individual cells.
This phenomenon, Dr Couzin argues, applies to all manner of organisms, from individual cells in a tissue to (rather worryingly) voters in the democratic process.
Tissue engineering involves the creation of multicellular tissues from individual cells.
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