Primary antibodies to the following markers from indicated sources were used at the indicated dilutions LAMP-1 (H4A3, mouse, Iowa Developmental Studies Hybridoma Bank 1∶1000) and LAMP-2 (H4B4, mouse, Iowa Developmental Studies Hybridoma Bank 1∶1000).
Methodology: R&D as a percent of sales taken from indicated sources.
The ImmunoCruz Staining System Kit (Santa Cruz Biotechnology, Santa Cruz, California, USA) and the DAB Chromogen Kit Vector Laboratoriess, Burlingame, California, USA) were purchased from indicated sources.
All cells were cultured under a humidified atmosphere of 5% CO2 at 37°C in RPMI 1640 medium (Sigma, St Louis, MO, USA) supplemented with 10% fetal bovine serum and were passaged for ⩽3 months before renewal from frozen, early-passage stocks obtained from the indicated sources.
Antibodies against CLIP-170 and HDAC6 (Santa Cruz Biotechnology), β-actin and HA (Sigma-Aldrich) and GFP (Roche) were obtained from the indicated sources.
Formaldehyde-D2 (28% in D2O, Cambridge Isotope Laboratory), formic acid and acetonitrile (Merck), and C18 desalting microcolumns (Nest Group) were from the indicated sources.
Antibodies against p62 (MBL PM045), β-actin (Sigma A5441), ATG5 (Sigma, A0856), parkin (Cell Signaling 2132, Millipore AB9244), FLAG (Sigma F1804) were from the indicated sources.
Antibodies against EB1 (BD Biosciences, San Jose, CA, USA), β-actin and α-tubulin (Sigma-Aldrich), and GFP (Roche, Indianapolis, IN, USA), and horseradish peroxidase-conjugated secondary antibodies (Amersham Biosciences, Chandler, AZ, USA) were obtained from the indicated sources.
The following materials were purchased from the indicated sources.
Adult female New Zealand White rabbits (Sommer, Wollmersdorf, Austria) and Balb/c mice (Institute of Biomedical Research, Medical University of Vienna, Austria) were obtained from the indicated sources.
Trolox (Cayman, Ann Arbor, MI, USA), JC-1 (Invitrogen), Mitosox-Red (Invitrogen), H2DCF (Invitrogen), NOX inhibitor III (EMD Millipore) were purchased from the indicated sources.
