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The success of this synthesis depends on the in situ reduction of Mn cations by carbon from GSs under hydrothermal conditions.
The researchers checked the brains of a couple of patients who had died from GSS and found that while they had none of the traditionally toxic PrPSc, a portion of their prions were in the PrPctm form, suggesting that the same mechanism is at work in both mice and man.
To analyse how individual trust varies in time with trust in their host country, we follow Aleksynska (2011) and add to our regressions the average value of trust in the host country in each year of observation (calculated from GSS's and HILDA's full samples).
Factors 1 and 2 from GSS were analyzed using linear regression models including age and affection status as covariates.
The curves resulting from GSS transformation are polygonal lines, which can still be characterized by a distinctive slope (Figure 1C and Figure S1).
The results show that CyPrP generated from GSS PrP mutants does not aggregate in the cytosol and thus, aggregation cannot be responsible for the loss of anti-Bax function in the GSS mutants that generate CyPrP.
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The polymorphism rate of the tested SSR markers was almost equal to or slightly higher than the corresponding rates (73.4/65.9, 57.9, 69.5%) for the previously developed SSR markers from GSSs (mainly BESs)/unique transcripts of B. napus, BACs of B. rapa and WGSs of B. oleracea.
This frequency is much higher than that in BAC-end sequences of B. rapa (one per 4.7 kb) [ 21] and B. napus (one every 4.0 kb) [ 22], and in the whole genome shotgun sequences of B. oleracea (one every 4.0 kb) [ 33], indicating that the frequency of SSRs identified from GSSs of B. rapa, B. oleracea and B. napus underestimated the abundance of microsatellites in Brassica genomes.
FC was analysed from one GSS case (P102L, small fragment), and Cb and FC was analysed from another GSS case (P102L, type 1).
These results show that the cytosolic PrP generated from the GSS mutants is not as efficient as wild type PrP in inhibiting Bax-mediated cell death.
NJK and MTC designed the study, NJK drafted the manuscript with assistance from MTC, GSS and CMR.
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