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However, since none of these two peptides elicited specific IFN-γ secretion among spleen cells (data not shown) they were excluded from further assays.
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Fifty-four formalin-fixed and paraffin-embedded (FFPE OSS tissues were obtained for FISH and IHC analysis (including nine aCGH analysis samples. One case which had aCGH data was excluded from further assay because of no enough sample).
The length of the colon was measured, the colon was flushed with cold PBS and opened longitudinally for morphologic studies, and tissue obtained from each colon was processed for further assays.
The collected serum was stored at –20°C for further assays.
The involvement of phytochromes, such as PHYA and PHYB, in the JA-light crosstalk can also be demonstrated from the further assays on OsJAR1 since the expression of this gene is down-regulated in both phyA and phyB rice mutants and completely eliminated in the phyA phyB double mutant (Riemann et al. 2008).
Discrete clones could then be isolated from these pools for further assay and final selection.
The remaining eluted DNA from COBAS 4800 will be stored at −80°C until required for further assay.
Therefore samples with low yield (< 5 μg) were excluded from further downstream assays.
Residual supernatants from stimulated PBMCs were stored at −80°C and subsequently, 65 samples from adults were further assayed in a Luminex xMAP 200 platform using customized bead preparations (Beadlyte Human 26-plex Multi-Cytokine, Millipore, Billerica, MA).
The RNA from the fingersticks were further assayed on Affymetrix GeneChips and the results were very similar to the venipuncture collections.
p = 0.009 The presence of genes for enterotoxins STa and LT was investigated by PCR on the same selected 55 strains from diarrhoeal calves and, since all the results were negative, the isolates from healthy calves were not further assayed for STa and LT genes.
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