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One of the advantages of zebrafish is that the oocyte is externally fertilized, allowing early developmental processes, from fertilization onwards, to be easily analyzed.
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From 3 d after fertilization onwards, reporter expression was observed in more organs and tissues, such as the liver, pancreas, pronephric ducts, intestine, olfactory placodes, cranial cartilages, and blood vessels.
From 2 d after fertilization onwards, we observed more lines with both reporters expressed in the CNS, especially strong in the eyes and specific sub-domains in the developing brain, as well as in other cell type-specific patterns, such as expression in the branchial arches, fins, otoliths, heart tube, notochord, hatching gland, and various muscle cell types.
From 24 hpf (hours-post fertilization) onwards, compared to control embryos, injected embryos showed reduced circulation of blood cells in the presence of a beating heart, albeit with lower beating rate (Figure 2A, Figure 2B, supporting information Figure S3 and Movie S1, S2, S3, S4, S5 and S6).
IL-1β and TNF-α transcripts were detected from 4 week post-fertilization onwards, whereas TGF-β transcript was detected only from 7 week post-fertilization onwards.
Transferrin and TLR22-like mRNA transcripts were detected by RT-PCR from 6 h post-fertilization to 31 day post-fertilization, whereas β-2 microglobulin transcripts were detected only from 7 day post-fertilization onwards.
Studies in larval zebrafish have previously demonstrated that LC3-I to LC3-II conversion occurs from 32 h post-fertilization onwards and that changes in LC3-II levels could be measured in the presence of lysosomal protease inhibitors (34).
The effects of rearing density and size grading on sex ratios in 30 families of sea bass reared in the same tank from the fertilization stage onwards were investigated.
In contrast, kif5aa mutant mRNA is dramatically down regulated in embryos starting from 24 hours post fertilization (hpf) onwards, probably by nonsense-mediated decay.
Loss-of-function alleles cell-autonomously lead to unpigmented melanophores in the body and in the retinal pigment epithelium, a phenotype that is readily visible in early larvae from 2 days post fertilization (dpf) onwards, as well as in adult zebrafish (Fig. 1C,D) (Kelsh et al., 1996; Streisinger et al., 1986).
Homozygous sapje zebrafish show a progressive loss of muscle organization visible from 3 days post-fertilization (dpf) onwards (6, 20).
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