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Briefly, total RNA from each tissue was isolated using the Trizol kit (Invitrogen).
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RNA from mouse tissue was isolated using TRIZOL (Invitrogen) according to standard protocols.
RNA from laser-captured tissue was isolated using the Absolutely RNA Nanoprep kit (Stratagene).
Total RNA from mouse tissues was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA).
RNA from adult mouse tissues was isolated using a commercial kit and protocol (Qiagen).
Total RNA from all 54 tissue specimens was isolated using Trizol kit (GIBCO BRL Life Technologies).
RNA was isolated using TRIzol reagent from the frozen mouse tissue according to manufacturer's instructions.
Total RNAs from tissue samples were isolated using Trizol Reagent (Invitrogen).
Tissue was isolated from whole kidney using a 5 mm biopsy punch with medullar tissue removed from the sample.
Total genomic DNA was isolated from tissue using the Qiagen DNAeasy Tissue Kit.
When necessary DNA was isolated from tissues using the method described by Pinto et al [31].
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