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So that we might understand the paths through which the system could possibly run, we ran the model in synchronous mode for 10 steps starting from each of the 2 (128) possible states of the model and mapped the dynamical trajectories from each start state to the final state via every state the model occupied on the way.
For the correlation and GO analyses, we extracted sequences 500 bp upstream from each start codon and truncated them to exclude any upstream coding sequences.
The sampling was coordinated in order to select and interview approximately equal numbers of participants across the selected wards, with equal numbers originating from each start point.
Construction of chromosomal sipA:: cyaA' and sseJ:: cyaA' was described previously [ 41]. pSrfN and pPagK2 expressing SrfN and PagK2 respectively were constructed by cloning DNA fragments containing 900 bp upstream sequences from each start codon and coding sequences of srfN or pagK2 on pWKS30 via EcoRI and XbaI.
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Place trees to block lines of sight TO each starting spot, but that do not block sight lines FROM each starting spot.
The winners from each starting point then reconvene in Monte Carlo where they race against each other.
The measure ({{mathrm{EPL}}}) was introduced by Hendrickson [8], and is defined as the sum of the numbers of reactions from each starting material to the target.
Ten structures were calculated from each starting structure.
From each starting tree, four related MCMC chains (one cold and three incrementally heated) were run.
Reaction components were sorted into two syringes, with rapid mixing of 60 μL from each starting each reaction.
Five replicate products from each starting quantity were precipitated with either 2.5 M LiCl, column, or guanidinium methods (figure 4D).
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